学位論文　北海道札幌市　旭川市　旭川駅近　買物公園　美容皮膚科　美容外科　皮膚科　形成外科｜旭川皮フ形成外科クリニック｜肥満治療（ダイエット方法他）　リンパ浮腫　ホルモン 補充療法　白斑　ニキビ治療

Mizuno H, Takeda A, Uchinuma E.
（Department of Plastic and Reconstructive Surgery, Kitazato University School of Medicine, Sagamihara, Kanagawa, Japan.）

As promising new therapy for congenital giant pigmented nevi, the authors investigated the potential use of an acellular autograft nevi-dermal matrix in combination with a split-thickness skin graft. To address whether the processed acellular nevi-dermal matrix from frozen skin could be reconstituted as a viable dermal base, the authors grafted it onto full-thickness skin defects in nude rats. Fibroblast infiltration and neovascularization into the acellular nevi-dermal matrix were observed. However, because the disappearance of the residual melanotic granules of the grafted dermis took 16 weeks, the authors excised with scissors the superficial layer of the acellular nevi-dermal matrix containing a large quantity of melanin. The appearance after using this method was relatively superior even compared with the full-thickness skin graft. The success of their experimental animal model using the acellular nevi-dermal matrix covered with split-thickness skin grafts confirms the potential value for the clinical application of this treatment for congenital giant nevi.


＜Ann Plast Surg. 1999 Sep;43(3):273-82.＞

Mizuno H, Takeda A, Uchinuma E.
（Department of Plastic and Reconstructive Surgery, Kitazato University School of Medicine, Kanagawa, Japan.）

At present, one of the treatments of choice for closure of full-thickness skin loss is to use a cultured epidermal autograft when skin loss is extensive. In this study, we investigated a simple method of processing frozen surplus skin to produce an acellular, structurally intact, dermal matrix. First, the acellular dermal matrix prepared from normal human skin (ADM) we processed was observed using a transmission electron microscope and a scanning electron microscope. The matrix maintained the basement membrane complex and the extracellular matrix structure of the dermis despite frozen skin being used. Next, using an animal model, we transplanted the ADM and Pelnac, which is used as a contrast in full-thickness wounds onto nude rats. The dermal matrix supported fibroblast infiltration and neovascularization. These results suggest that skin processed by our simple method has the potential to be used as a dermal template together with the cultured epidermis in the closure of full-thickness wounds.

＜Aesthetic Plas Surg. 1999 Sep-Oct;23(5):316-22.＞